Standard Check Calculator
Confirm that two independently weighed standard preparations agree before you use them, corrected for their weights and dilutions.
- 98.0–102.0% default
- HPLC and UV-Vis
- Weight-corrected
- No sign-up
Four steps, or fewer
- Enter the weight and dilution volume of both standard preparations.
- Paste the replicate responses of standard 1 and the responses of standard 2.
- Read the standard check result and the %RSD of standard 1.
Not sure what to enter? Press Load example to see the calculator with realistic data. Your inputs are saved in this browser, so they are still there when you come back.
How the standard check works
Two standard solutions are prepared from separate weighings. Each mean response is divided by its concentration to give a response factor; the ratio of the two factors shows whether the preparations agree.
Standard check (%) = RF₂ / RF₁ × 100
Both standards are assumed to come from the same reference standard lot, so purity cancels out. A common acceptance range is 98.0–102.0%, together with ≤ 2.0% RSD for the replicate injections of standard 1. Use the limits in your SOP.
References
- USP General Chapter <621> Chromatography
- Your laboratory SOP for standard preparation
These calculators are decision support for orientation and training. They are not validated GMP software, and results must be checked against your approved method, SOP and the current pharmacopoeia or guideline before use in GMP records. Your data is processed in your browser and is not sent to Molgenium.